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Seminars

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  • Title:Supported Plasma Membrane Platforms from Giant Plasma Membrane Vesicles to Study Membrane Proteins
  • Start Date/Time:2017-12-14 / 12:00
  • End Date/Time :2017-12-14 / 14:00
    • Speaker:Prof Ling Chao (NTU)
    • Place:P512 of NCTS, 5F, 3rd General Building, Nat'l Tsing Hua Univ.
    • Host:Prof. Lee-Wei Yang (NTHU)
    • Abstract:
      Studying cell membrane proteins while maintaining their intact structural information remains one of the largest bottlenecks to characterizing and understanding the protein structure-function behaviors. Our lab obtained giant plasma membrane vesicles (GPMVs) directly from Hela cells and used them to form supported lipid bilayer platforms, so that the membrane proteins can be processed in their native lipid bilayer environment. We demonstrated that not only lipids but also a native transmembrane protein in HeLa cells, Aquaporin 3 (AQP3), is mobile in the supported membrane platform. The region of the support which was not covered by GPMV patches can be filled with artificially prepared lipid vesicles to form a continuous supported membrane for the protein collection purpose. The method for generating a continuous supported membrane platform with mobile native transmembrane proteins could not only facilitate the study of membrane proteins by surface analytical tools, but could also enable us to use native membrane proteins for bio-sensing applications.
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