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Seminars

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  • Title:Serum-free expansion, induction and pre-clinical application of human hematopoietic stem cells
  • Start Date/Time:2018-04-17 / 12:00
  • End Date/Time :2018-04-17 / 13:20
    • Speaker:Prof. Chao-Ling Yao (YZU)
    • Place:Lecture Room A of NCTS, 4F, General Building III, NTHU
    • Host:Prof. Lee-Wei Yang (NTHU)
    • Abstract:
      Cord blood (CB) has been identified as a rich source of hematopoeitic stem cells (HSCs) available for transplantation. Since the first case of Fanconi’s anemia patient received successful CB transplantation in 1988, more than 6,000 patients have been performed. The advantages of using CB as HSC sources comparing with traditional bone marrow are following: 1) There is no harm to the donor. 2) There is significantly reduced level of graft-verse-host disease. 3) There is low contamination rate/ risk with common viruses, such as CMV and EBV. 4) Mature processing and banking system for storage and management. 5) Cost efficient for transplantation process. However, the limitation of CB transplantation is the insufficient cell number in the collected CB unit.
      In order to solve the shortage of cell number in CB, we developed an optimized serum-free medium for ex vivo expansion of HSCs from UCB. Besides, the serum-free expanded HSCs were further to be induced into natural killer (NK) cells, megakaryocytes (MKs), macrophage and red blood cells specifically. Here, we would briefly introduce the results of the serum-free expanded HSCs and their differentiated cells according to their future applications, such as HSC transplantation, cell therapy, immunotherapy, cell-based test platform and humanized mice establishment, respectively.
    • Detailed contents: 
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